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2018GXNSFAA281153), and National Natural Science Foundation of China (No

Following treatment, the medium was removed, cells were washed 2 times with warm PBS, and fixed with 4% formaldehyde for 20 min

For subcutaneous injections, both are effective for systemic delivery

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This is based on the selectivity and high affinity of Ni-NTA(nickel nitriloacetic acid) reins for proteins containing his tag